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. 2019 Aug 21;20(10):e48014. doi: 10.15252/embr.201948014

Figure EV5. UBE2QL1 is essential for maintenance of lysosomal integrity (related to Fig 7).

Figure EV5

  1. Immunofluorescence images for UBE2QL1 siRNA #5 corresponding to Fig 7A, with the first row of control cells being identical. Scale bar: 20 μm.
  2. Immunofluorescence images for UBE2QL1 siRNA #5 corresponding to Fig 7C, with the first row being identical. Scale bar: 20 μm.
  3. Rescue of the effects of UBE2QL1 depletion on lysosomal homeostasis by UBE2QL1 overexpression. Lysates from rescue experiments in Fig EV2C were probed with antibodies against TFEB, LAMP1, and pS6 to monitor the effects of overexpression of UBE2QL1 WT and CS mutant in UBE2QL1‐depleted cells. The blots for UBE2QL1 and GAPDH (identical to Fig EV2C) are shown for clarity. Lysates used for all blots are from the same experiment. The asterisk indicates an unspecific band.

Source data are available online for this figure.