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. 2019 Aug 12;20(10):e47472. doi: 10.15252/embr.201847472

Figure EV2. Over‐expression of DHHC5 causes a localisation defect.

Figure EV2

  1. Confocal images of endogenous DHHC5 in HeLa cells.
  2. Confocal images of HeLa cells expressing WT DHHC5 C‐terminally tagged with a FLAG epitope.
  3. Confocal images of HeLa cells expressing a C‐terminally FLAG‐tagged catalytic mutant of DHHC5 where the active site cysteine of DHHC5 has been replaced with a serine (DHHS5).
  4. Confocal microscopy images of HeLa cells expressing DHHC5 with the three palmitoylated cysteines in the C‐terminal tail mutated to serine residues. Antibody detecting endogenous FLAG epitope.
  5. Quantification of membrane signal of DHHC5 seen in (A‐D). All experiments were repeated three individual times; all n numbers refer to the number of cells quantified. Endogenous DHHC5, n = 45; DHHC5, n = 48; DHHS5 only, n = 38; C‐terminal mutant, n = 35, ****P < 0.001, unpaired t‐test. Error bars represent SEM.
Data information: All scale bars: 10 μm.