Figure EV5. Prostate orthotopic xenograft of DU145 cells with CTH overexpression induces tumor growth and incidence of paraaortic lymph node metastasis.

• A
  Western blot analysis of the CTH expression in DU145 cells stably expressing PCDNA or PCDNA‐CTH.
• B–D
  1 × 10⁶ DU145 cells with PCDNA or PCDNA‐CTH expression were orthotopically injected into the mouse prostate for 30 days. The mouse weight (B) and tumor volume (C) were compared. Incidences of paraaortic lymph node metastasis (D) are shown. Data in (B, C) are presented as means ± SEM (n = 7 or 8 mice per group). Student's t‐test was used for the statistical analysis (*P < 0.05).
• E, F
  Images of the orthotopic xenograft tumor (E) and paraaortic lymph nodes (F) of mice orthotopically implanted with DU145‐PCDNA or CTH cells.
• G
  Images show H&E staining of paraaortic lymph node sections. T: metastatic tumor cells (scale bar: 10 μm).

Source data are available online for this figure.