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. 2019 Aug 29;20(10):e48111. doi: 10.15252/embr.201948111

Figure EV5. Effect of H3K9me on CLRC‐mediated ubiquitylation.

Figure EV5

Ubiquitylation assays using biotinylated ubiquitin and recombinant wild‐type and mutant human H3.2, and the K9me3 analog of human H3.2 (C110A K9Cme3). The proteins were resolved by SDS–PAGE and analyzed by Western blotting (left and center). The recombinant protein substrates were also analyzed by Coomassie Brilliant Blue (CBB) staining (right). The single and double asterisks indicate mono‐ and di‐ubiquitylated H3.2, and the triple asterisks indicate cross‐linked dimers of human H3.2 C110A K9C.