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. 2019 Aug 23;20(10):e47911. doi: 10.15252/embr.201947911

Figure EV1. Prevention of IO‐induced insulin resistance in L6 cells with iron chelator.

Figure EV1

  1. MTT viability assay in L6 cells after iron treatment (FeSO4, 250 μM) for 24 h.
  2. Time course measurement of intracellular iron concentration in L6 cells after iron treatment (250 μM) with iron chelator DPD (500 nM) for multiple time points. # P < 0.05 (unpaired Student's t‐test versus iron at each time points).
  3. Glucose uptake of L6 cells with insulin stimulation (100 nM, 20 min) after iron treatment (250 μM, 24 h) with DPD (500 nM). *P < 0.05, # P < 0.05 (one‐way ANOVA test with multiple comparisons).
  4. Representative Western blot images of phosphor‐IRS1 Y612, phosphor‐AKT T308, and GAPDH expression.
  5. Quantification of phosphor‐IRS1 Y612 over GAPDH and phosphor‐AKT T308 over GAPDH in L6 cells with insulin stimulation (100 nM, 5 min) after iron treatment (250 μM, 24 h) with DPD (500 nM). *P < 0.05 (unpaired Student's t‐test versus basal without insulin), # P < 0.05 (unpaired Student's t‐test versus iron with insulin).
Data information: All experiments were performed three times. Results are represented as mean ± SEM.