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. 2019 Sep 20;4(14):15966–15974. doi: 10.1021/acsomega.9b02010

Figure 3.

Figure 3

Oenothein B inhibits NF-κB-driven transcription and nuclear translocation induced by TNF-α or IL-1β in AGS cells. AGS cells were transiently transfected with the NF-κB-LUC plasmid and then treated with increasing concentrations of oenothein B in the presence of TNF-α (panel A) or IL-1β (panel B). Treatment with the reference compound (20 μM EGCG) reduced transcription by 85 and 64% for TNF-α and IL-1β, respectively. The amount of p65 in the nuclear fraction following treatment with oenothein B in the presence of the proinflammatory stimuli for 1 h was also assessed (panels C and D). Treatment with the reference compound (20 μM EGCG) reduced translocation by 90 and 64% for TNF-α and IL-1β, respectively. The graphs show the means ± sd of at least three experiments. Statistical analysis: one-way analysis of variance (ANOVA), followed by Bonferroni as the post hoc test. *p < 0.05, **p < 0.01, ***p < 0.0001 versus stimulus alone.