KEY RESOURCE TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Sheep polyclonal anti-Amylase | BioGenesis | 0480–0104 |
| Goat polyclonal anti-Cpa1 | R&D Systems | AF2765 |
| Rabbit monoclonal anti-Cleaved-caspase3 | Cell Signaling | 9664S |
| Rabbit monoclonal anti-Claudin-18 | Invitrogen | 700178 |
| Rat monoclonal anti-Cytokeratin-19 | Developmental Studies Hybridoma Bank (DSHB) | TROMA-III |
| Rabbit monoclonal anti-Cytokeratin-19 | Abcam | AB133496 |
| Chicken polyclonal anti-GFP | Aves Labs | GFP-1010 |
| Rabbit polyclonal anti-GFP | Abcam | Ab290 |
| Mouse monoclonal anti-Ki67 | BD Biosciences | 550609 |
| Rabbit polyclonal anti-Ptf1a | Chris Wright, Vanderbilt University | N/A |
| Rabbit polyclonal anti-phospho-ERK1/2 (T202/Y204) | Cell Signaling | 9101 |
| Rabbit polyclonal anti-Sox9 | Millipore | AB5535 |
| Mouse monoclonal anti-β-tubulin | DSHB | 12G10 |
| Chemicals, Peptides, and Recombinant Proteins | ||
| Tamoxifen | Cayman Chemical | 13258 |
| Doxycycline | Research Products International |
D43020–25.0 Lot 22362 |
| Zinc formalin fixative | Anatech | 174 |
| Paraformaldehyde 16% Solution | Electron Microscopy Sciences | 15710 |
| Caerulein | Bachem | 4030451 |
| Deposited Data | ||
| Normal human pancreas RNA-seq data (TPM), GTEX | GTEx Portal | https://storage.googleapis.com/gtex_analysis_v7/rna_seq_data/GTEx_Analysis_2016-01-15_v7_RNASeQCv1.1.8_gene_median_tpm.gct.gz |
| Normal human pancreas RNA-seq data (TPM), Human Protein Atlas | EMBL-EBI Expression Atlas | https://www.ebi.ac.uk/gxa/experiments/E-MTAB-2836/Downloads?ref=aebrowse |
| Human cancer cell line RNA-seq data (TPM), Genentech | EMBL-EBI Expression Atlas | https://www.ebi.ac.uk/gxa/experiments/E-MTAB-2706/Downloads?ref=aebrowse |
| Human cancer cell line RNA-seq data (TPM), Cancer Cell Line Encyclopedia | EMBL-EBI Expression Atlas | https://www.ebi.ac.uk/gxa/experiments/E-MTAB-2770/Downloads?ref=aebrowse |
| Experimental Models: Cell Lines | ||
| HEK 293T | ATCC | CRL-3216 |
| ASPC1 | ATCC | CRL-1682 |
| HPAFII | ATCC | CRL-1997 |
| Panc1 | ATCC | CRL-1469 |
| MiaPaCa2 | ATCC | CRL-1420 |
| Su8686 | ATCC | CRL-1837 |
| SW1990 | ATCC | CRL-2172 |
| Experimental Models: Mouse Strains | ||
| Mouse: Ptf1aCreERTM (Ptf1atm2(CreER1)CVW) | Jackson Laboratory | 019378 |
| Mouse: KrasLSL-G12D (Krastm4tyj) | Jackson Laboratory | 008179 |
| Mouse: Rosa26rtTA-EGFP (B6.Cg-Gt(ROSA)26Sortm1(rtTA,EGFP)NagyI) |
Jackson Laboratory | 005670 |
| Mouse: tetO-Ptf1a | Raymond MacDonald, UT-Southwestern | N/A |
| Oligonucleotides | ||
| see Table S2 | ||
| Recombinant DNA | ||
| pENTR221-mPtf1a | this paper | N/A |
| pME-EGFP | (Kwan et al., 2007) | N/A |
| pCLX-pTF-R1-DEST-R2-EBR65 | Addgene | # 45952 |
| pCLX-pTF-mPtf1a | this paper | N/A |
| pCLX-pTF-EGFP | this paper | N/A |
| psPAX2 | Addgene | # 12259 |
| pCAG-VSVG | Addgene | # 35616 |
| Other | ||
| Citrate-based Antigen Unmasking Solution | Vector Laboratories Inc | H-3300 |