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. 2019 Oct 3;7:2515135519874677. doi: 10.1177/2515135519874677

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© The Author(s), 2019

This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 License (http://www.creativecommons.org/licenses/by-nc/4.0/) which permits non-commercial use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access pages (https://us.sagepub.com/en-us/nam/open-access-at-sage).

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Figure 3. — The proliferation of HCV-specific T cells upon in vitro restimulation. Splenocytes isolated from SFV immunized mice were labeled with CFSE and cultured in the presence (+) or absence (–) of irradiated Hepa1-6^VenusnsPs cells at a 25:1 ratio for 4 days. Subsequently, the percentage of proliferating cells was analyzed by flow cytometry after staining with anti-CD8a. The bars represent the percentage of proliferating daughter CFSE⁺CD8⁺ cells (sum of generation 1–7) within the total CFSE⁺CD8⁺ cells (sum of generations 0–7). Data represent mean ± SEM (n = 3–4).