Table 2. Crystallization conditions.
Method | Sitting-drop vapor diffusion |
Plate type | 48-well |
Temperature (K) | 293 |
Protein concentration (mg ml−1) | 10 |
Buffer composition of protein solution | 5 mM Tris–HCl pH 8.0, 100 mM NaCl |
Composition of reservoir solution | 0.2 M sodium potassium tartrate tetrahydrate, 0.1 M sodium citrate tribasic dihydrate, 2.0 M ammonium sulfate pH 5.6 |
Volume and ratio of drop | 2 µl; 1:1 ratio |
Volume of reservoir (µl) | 70 |