Skip to main content
. 2019 Sep 20;75(Pt 10):634–639. doi: 10.1107/S2053230X1901238X

Table 2. Crystallization conditions.

Method Sitting-drop vapor diffusion
Plate type 48-well
Temperature (K) 293
Protein concentration (mg ml−1) 10 
Buffer composition of protein solution 5 mM Tris–HCl pH 8.0, 100 mM NaCl
Composition of reservoir solution 0.2 M sodium potassium tartrate tetrahydrate, 0.1 M sodium citrate tribasic dihydrate, 2.0 M ammonium sulfate pH 5.6
Volume and ratio of drop 2 µl; 1:1 ratio
Volume of reservoir (µl) 70