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. 2019 Oct 1;13(2):131–136. doi: 10.1055/s-0039-1694904

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This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivatives License, which permits unrestricted reproduction and distribution, for non-commercial purposes only; and use and reproduction, but not distribution, of adapted material for non-commercial purposes only, provided the original work is properly cited.

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Fig. 1 — Evaluation of cell morphology, viability, and alkaline phosphatase activity. ( A ) Evaluation of cell morphology on day 1 using inverted microscopy following treatment with different concentrations of dimethyl sulfoxide (0%, 0.01%, 0.1%, 1.0%, 3.0%, and 10.0%) on osteogenic supplement. The bar indicates 400 μm. ( B ) The CCK-8 assay results on day 1 cultured with osteogenic media. ( C ) The CCK-8 assay results on day 3. ( D ) The CCK-8 assay results on day 5. ( E ) The CCK-8 assay results on day 7. ( F ) Alkaline phosphatase activity on days 3, 7, 10, 14, and 21. CCK-8, cell counting kit-8.