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. 2019 Oct 1;13(2):131–136. doi: 10.1055/s-0039-1694904

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This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivatives License, which permits unrestricted reproduction and distribution, for non-commercial purposes only; and use and reproduction, but not distribution, of adapted material for non-commercial purposes only, provided the original work is properly cited.

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Fig. 2 — Alizarin red S staining and quantitative RT-PCR results. ( A ) Results of alizarin red S staining on day 5 following treatment with different concentrations of dimethyl sulfoxide (0%, 0.01%, 0.1%, 1.0%, and 3.0%) on osteogenic supplement. The bar indicates 200 μm. ( B ) Quantitative results of alizarin red S staining on days 5, 14, and 21. ( C ) Quantitative RT-PCR results of Runx2 expression on day 11 on osteogenic media. ( D ) Quantitative RT-PCR results of collagen I expression on day 11 on osteogenic media. RT-PCR, real-time polymerase chain reaction.