Table. Summary of Clinical Information.

Feature	Case 1	Case 2	Case 3	Case 4
First tumora
Largest diameter, mm	15	10	15	11
Thickness, mm	3.5	2.3	3.9	7.8
Vitreous dispersion of melanoma cells at presentation	No	No	No	Localized
Postradiation recurrence	No	Yes	No	No
Second tumorb
Treatment	Enucleation	Enucleation	Transpupillary thermotherapy cryotherapy	Enucleation
Time measurements
To detection, moc	20	18	21	43
To metastasis, moc	32	25	NA	NA
To overall follow-up, moc	37	25	26	48
Disposition
Last status	Alive with metastasis	Dead of metastasis	Alive without metastasis	Alive without metastasis
Genes mutated in first and subsequent tumors	GNAQ Q209L, BAP1 R207fs	GNA11 Q209L	GNAQ Q209L, BAP1 R244fs	GNA11 Q209L, BAP1 E198fs

Abbreviations: BAP1, BRCA1-associated protein 1; GNA11, guanine nucleotide-binding protein subunit alpha-11; GNAQ, G protein subunit alpha Q; NA, not applicable.

^aIn all cases, first tumors did not have ocular or oculodermal melanocytosis or ciliary body involvement; all were treated with plaque radiotherapy.

^bIn all cases, second tumors were found to have class 2 tumors on gene expression profiling.

^cThe starting point for all time measurements is the initial primary tumor treatment.