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. 2019 Aug 29;18(5):3475–3483. doi: 10.3892/etm.2019.7960

Figure 3.

Figure 3.

XIST directly targets miR-133a in bladder cancer cells. (A) Bioinformatics analysis suggested that miR-133a has putative XIST binding sites, and luciferase reporter plasmids containing the WT and MT miR-133a binding sites in XIST were generated. (B) Knockdown of XIST significantly promoted miR-133a expression in 253J and RT112 cells. **P<0.01 vs. siNC. (C and D) Luciferase reporter gene assay suggested that transfection with miR-133a mimics decreased the luciferase activity of WT XIST reporter plasmid, while not affecting the luciferase activity of MT XIST reporter plasmid in 253J and RT112 cells. **P<0.01 vs. miR-NC. XIST, X inactive specific transcript; miR, microRNA; miR-NC, miR mimics negative control; WT, wild type; MT, mutant type; siRNA, small interfering RNA; siNC, negative control siRNA; siXIST, siRNA targeting XIST.