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. 2019 Aug 29;18(5):3475–3483. doi: 10.3892/etm.2019.7960

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Copyright: © Zhou et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 5. — miR-133a acts as a downstream effector in XIST-mediated bladder cancer cell proliferation and migration. 253J and RT112 cells were co-transfected with NC siRNA and NC inhibitor, NC siRNA and miR-133a inhibitor, XIST siRNA and NC inhibitor or XIST siRNA and miR-133a inhibitor, respectively. (A and B) The expression of XIST was examined using reverse-transcription quantitative PCR. (C and D) A Cell Counting Kit-8 assay and (E and F) a wound healing assay (magnification, ×40) were performed to assess cell proliferation and migration, respectively. **P<0.01 vs. si-NC+anti-NC. XIST, X inactive specific transcript; miR, microRNA; anti-miR-133a, miR-133a inhibitor; anti-NC, miR inhibitor control, siRNA, small interfering RNA; siNC, negative control siRNA; siXIST, siRNA targeting XIST; OD, optical density.