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. 2019 Aug 30;18(5):3525–3533. doi: 10.3892/etm.2019.7962

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Copyright: © Ma et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 3. — miR-205-5p negatively regulates ERp29 in breast cancer cells. (A) miR-205-5p inhibitor significantly inhibited miR-205-5p expression in the MDA-MB-231 cell line. (B) RT-qPCR results indicated that miR-205-5p inhibitor enhanced ERp29 mRNA expression levels in the MDA-MB-231 cell line. Protein expression levels of ERp29 were determined using (C) western blotting and (D) quantified. (E) RT-qPCR results indicated that miR-205-5p mimic decreased ERp29 mRNA expression levels. Protein expression levels of ERp29 in the MDA-MB-231 cell line were determined using (F) western blotting and (G) analyzed quantitatively. **P<0.01 and ***P<0.001 vs. the respective control group. RT-qPCR, reverse transcription-quantitative PCR; NC, negative control; miR, microRNA; ERp29, endoplasmic reticulum protein 29.