Figure 4.

miR-205-5p regulates ERp29 by binding to its 3′-UTR. (A) Bioinformatic prediction of the putative binding site of miR-205-5p on the 3′-UTR of ERp29. (B) Dual-luciferase assay confirmed that the luciferase activity was reduced in MDA-MB-231 cells co-transfected with miR-205-5p mimic and WT-ERp29-3′-UTR reporter plasmid and was unaltered in cells co-transfected with miR-205-5p mimic and Mut-ERp29-3′-UTR reporter plasmid. ***P<0.001 vs. the miR-NC mimic. 3′-UTR, 3′untranslated region; WT, wild-type; Mut, mutant; NC, negative control; miR, microRNA; ERp29, endoplasmic reticulum protein 29.