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. 2019 Aug 30;18(5):3525–3533. doi: 10.3892/etm.2019.7962

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Copyright: © Ma et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 6. — Overexpression of miR-205-5p reverses gemcitabine resistance of MDA-MB-231/GEM cells via repression of ERp29. Compared with the miR-NC mimic + pcDNA3.1 group, protein expression level of ERp29 decreased in the miR-205-5p mimic + pcDNA3.1 group and this effect was reversed by transfection with miR-205-5p mimic + pcDNA3.1-ERp29 as demonstrated by (A) western blotting and (B) quantitative analysis. Cell apoptosis of the transfected cells was analyzed by (C) flow cytometry and (D) quantified. (E) Cell viability was measured using Cell Counting Kit-8. **P<0.01 and ***P<0.001. NC, negative control; miR, microRNA; ERp29, endoplasmic reticulum protein 29; PI, propidium iodide.