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. 2019 Sep 9;18(5):3650–3658. doi: 10.3892/etm.2019.7989

Figure 3.

Figure 3.

MACC1 is a direct target of miR-877 in cervical cancer cells. (A) The two predicted binding sites of miR-877 in the 3′-UTR of MACC1. The mutant binding sites were produced in the seed region of the MACC1 3′-UTR by mutating 7 nucleotides that were targeted by miR-877. (B) The luciferase reporter assay was performed in HeLa and SiHa cells that were co-transfected with miR-877 mimics or miR-NC and luciferase reporter plasmid carrying the wt or mut miR-877 binding site. *P<0.05 vs. miR-NC. (C) Detection of MACC1 mRNA expression in 57 pairs of cervical cancer tissues and matched adjacent normal tissues was performed via RT-qPCR. *P<0.05. (D) Spearman's correlation analysis was used to determine the association between the expression levels of miR-877 and MACC1 mRNA in cervical cancer tissues. r=−0.5618, P<0.0001. MACC1 mRNA and protein expression in miR-877 mimic- or miR-NC-transfected HeLa and SiHa cells was detected via (E) RT-qPCR and (F) western blot analysis, respectively. *P<0.05 vs. miR-NC. MACC1, metastasis-associated in colon cancer 1; miR, microRNA; 3′-UTR, 3′-untranslated region; wt, wild type; mut, mutant; RT-qPCR, reverse transcription-quantitative PCR; NC, negative control.