Table I.
Gene | Primer sequence (5′-3′) |
---|---|
Bcl-2 | F: 5′-CACAAGAGGCCAAGGCTACC-3′ |
R: 5′-CAGGAAAGCAGGAAGTCTCAA-3′ | |
Bcl-xl | F: 5′-CCAAAATCCCTGCTCTTCATG-3′ |
R: 5′-GCATTCTTGGCATCGTTATTCA-3′ | |
Bax | F: 5′-ATTGAGAAACGATTTGCCTAC-3′ |
R: 5′-GGAAATGGCTTATTCTCCTTTGCTT-3′ | |
Bad | F: 5′-GGAAACCCGGTGGGGCCAC-3′ |
R: 5′-ACCAGTAGCGGGTGGTC-3′ | |
Iba-1 | F: 5′-CACCATTAGCTGGGCGTCT-3′ |
R: 5′-GATGCGGAAGTAGCAAAAGC-3′ | |
MPO | F: 5′-ATTCTCCACACCCTGTTTCG-3′ |
R: 5′-ATGCAGCAGTGTGTCATTCC-3′ | |
TGF-β | F: 5′-GGCCAGATCCTGTCCAAGC-3′ |
R: 5′-GTGGGTTTCCACCATTAGCAC-3′ | |
ERK | F: 5′-ACCTAGCCGTGGAGCTTGG-3′ |
R: 5′-GCCCTTGGTTGTTTACCTGG-3′ | |
β-actin | F: 5′-AAGGACCTGTATGCCAACACA-3′ |
R: 5′-ATCCACACAGAATACTTGCGTT-3′ |
Bcl-2, B cell lymphoma-2; Bcl-xl, B cell lymphoma-extra large; Bax, Bcl-2-assocaited X protein; Bad, Bcl-2-associated death promoter; Iba-1, ionized calcium binding adaptor molecule 1; MPO, myeloperoxidase; TGF-β; transforming growth factor-β; ERK, extracellular signal-regulated kinase.