TABLE 2.
List of primers used for the gene expression analysis in the present study.
| Gene | Primer sequence (5′–3′) | Annealing temperature (°C) | Primer efficiency | Amplicon size (bp) | GenBank number |
| ef-1a | F-TCTTGGTCGTTTTGCTGTGC R-AGCCTTGATGACACCGACAG |
60 | 1.8 | 61 | AF321836 |
| actin | F-TCAGGGAGTGATGGTTGGGA R-GCCACTCTCAGCTCGTTGTA |
60 | 2.0 | 171 | NM_001123525.1 |
| tnf–a | F-ACACACTGGGCTCTTCTTCG R-GCACTTGACCCTAAACGAAGC |
58 | 2.0 | 52 | NM_001123589.1 |
| pcna | F-ACAGTTGTGTGGTCAGGATGC R-GAACTTAACGCCATCCTTGG |
60 | 1.9 | 110 | BT056931 |
| hsp-70 | F-TGGTCCTGGTGAAGATGAGG R-TGGCCTGTCTCTGTGAATCG |
60 | 1.9 | 108 | AJ632154 |
| tlr-3 | F-CTCTAACGGCAACCAGAAGC R-ATGGTGAGGTTGGACAGAGG |
60 | 2.0 | 144 | BK008646 |
| mx-1 | F-AAGCTGGCAGAGACACATGC R-ACATCCTTTCTGCCGAGTCC |
60 | 1.9 | 73 | NM_001123693 |