TABLE 4.

Alpha diversity of the distal intestinal microbiota comparing probiotic and control groups and SWT effect in the mucosa and digesta.

						Two-ways ANOVA model/linear mixed model
	Alpha diversity					Water				Water habitat ×
	index1	FW		SW		habitat		Diet		Diet
Tissue		Control	Probiotic	Control	Probiotic	f	P	f	P	f	P
Digesta	PD	11.3 ± 1.1	10.4 ± 0.8	10.7 ± 0.6	9.4 ± 0.7	2.85	0.13	5.57	0.046	0.16	0.7
(n = 3)	Shannon1	5.3 ± 0.3ab	5.1 ± 0.2ab	5 ± 0.1a	5.8 ± 0.4b	0.99	0.35	3.50	0.010	10.8	0.011
Mucosa2	PD	9.4 ± 1.2a	9.9 ± 1.2a	8.8 ± 1a	6.5 ± 1b	3.71	0.002	1.93	0.126	35.87	<0.001
(n = 6)	Shannon	6 ± 0.2	5.9 ± 0.3	6.2 ± 0.4	5.2 ± 0.4	1.78	0.093	4.31	0.012	95.64	<0.001

FW, freshwater; SW, seawater; PD, phylogenetic diversity. ¹Values are means ± SD, letters are present when a significant difference was identified by two-ways ANOVA model and post hoc test (Tukey HSD). Means in a row without a common letter differ, P < 0.05. Alpha diversity was calculated from samples rarefied at an even depth of 6,435 sequences. ²A linear mixed model was used to evaluate statistical differences in bacterial microbiota of mucosa with Diet and Water habitat as fixed factors and Tank as a random factor.