Figure 6.
Inhibition of miR-328 impedes tumor growth and metastasis in the orthotopic ovarian xenograft model. A, Representative images of orthotopic ovarian xenografts generated by injecting 2008-MIZP328–3p cells into the ovary of nude mice. Volumes of xenografts in mice treated or untreated with cumate were measured. **, P < 0.01. B, Macroscopic appearances and counts of ovarian metastasis nodules in peritoneal cavity. C, Representative H&E-stained images of the xenograft tumors and metastasis nodules. Bar, 100 μm. D and E, Xenotransplantation limiting dilution assay was used to estimate TICs in these xenografts. Tumor cells were isolated from xenografts and injected into NOD/SCID mice subcutaneously in a limiting dilution manner. Images of tumors after 2 weeks of injection were presented, and the number of xenografts was counted to generate TICf using the online algorithm described in Materials and Methods (D). Weight of tumors generated with 1 × 106 xenograft cells isolated from control and cumate-treated mice bearing orthotopic xenografts (E). F, Model of the mechanism by which miR-328 regulates the maintenance of CSC properties and tumor metastasis.