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(A) confocal micrograph; (B) automated pore identification for size and aspect ratio measurements using the Imaris (8.4.1) software; (C) image mask created based on absolute intensity used for image-sbased porosity, pore size (area and axes), and cell wall thickness measurements; (D) object-oriented method (in blue box) for long and short axes measurements for each pore, which is independent of image orientation (adapted from Imaris 8.4.1).
