Figure 6.

Accumulation of monoclonal Ab vs Ab cocktail in the joints of BALB/c mice after iv injection: (A) epitope of CB11 domain of collagen II targeted by the CB11 monoclonal antibody; (B) NIR image of SDS-PAGE gel of both CB11–800 and Arthrogen-800 showing similar labeling efficiency (100 ng of each Ab was loaded); (C) collagen binding assay shows binding to collagen with similar affinity but lower efficiency for CB11–800; (D) images of bones exposed to CB11–800 and Arthrogen-800 ex vivo and quantification of mean fluorescence of joints at 800 nm; (E) CB11 and Arthrogen show similar rates of clearance determined by plasma fluorescence at 800 nm; (F) images of dissected bones of CB11–800 and Arthrogen-800 iv-injected mice. HL = hind limb, FL = forelimb, and S = spine. The signal in 700 nm (autofluorescence) was enhanced to make the bones visible; (G) quantification of total fluorescence of joints in (F) shows higher accumulation of Arthrogen-800 in most joints.