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Expression of HE4 in ovarian cancer cells. mRNA (A) and protein (B) expression levels of HE4 were detected by qRT-PCR and western blot assays, respectively. qRT-PCR was used to evaluate the levels of HE4 in OVCAR3 (C) and C13K (D) cells transfected with sh-HE4-1, sh-HE4-2, or sh-Ctrl. Western blot assays were conducted to measure the protein levels HE4 in transfected OVCAR3 (E) and C13K (F) cells. *P<0.05.
