Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2019 Sep 15;8(9):bio047175. doi: 10.1242/bio.047175

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2019. Published by The Company of Biologists Ltd

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.

PMC Copyright notice

Fig. 1. — Live imaging of endogenously tagged proteins involved in centriole duplication. (A) Time course of centriolar Plk4-mClover fluorescence from a single cell. The cell divided twice during the 30 h observation period, as indicated by the arrows showing metaphase. Schematics in the graph show putative spatial patterns of Plk4 around the mother centriole at the corresponding time points. The endogenous tagging system is schematically shown on the right. (B–D) Averaged time courses of Plk4-mClover (B), STIL-mCherry (C) and HsSAS6-mCherry (D) signals at the centrioles of 14, 11 and 12 cells, respectively. Time course data were aligned at metaphase (0 h). The period between two metaphase time points is defined as one cycle. Note that the length of the cycle varies slightly among the averaged graphs due to the variety in the cell population. Representative images are shown on the left of each graph. Error bars, s.d. A.U., arbitrary units. Scale bar: 10 μm.