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. 2019 Sep 27;10:1111. doi: 10.3389/fphar.2019.01111

Figure 5.

Figure 5

Effect of OA and UA on the UGT1A1 reporter gene in HepG2 cells transiently transfected with hPXR. The HepG2 cells were transfected with pcDNA 3.1 (+)-hPXR, pcDNA 3.1 (+), pGL3-UGT1A1-luc, and/or pGL3-Basic, together with pRL-TK control vector. After transfection for 24 h, the transfected HepG2 cells were cultured with RIF (10 μM) or vehicle DMSO (0.1%) for an additional 24 h (A). The HepG2 cells were transiently co-transfected with pcDNA 3.1 (+)-hPXR expression vector, pGL3-UGT1A1-luc reporter vector, and pRL-TK control vector. After transfection for 24 h, the transfected HepG2 cells were cultured with OA (10, 20, and 40 μM), UA (10, 20, and 40 μM), RIF (10 μM), or DMSO (0.1%) for an additional 24 h (B). Luciferase activity was measured using the dual-luciferase reporter assay system. The transfection efficiency was normalized against renilla luciferase activity from the co-transfected pRL-TK vector. DMSO (0.1%) was used as the negative control. RIF (10 μM) was used as the positive control. The data are expressed as the mean ± SD of triplicate independent experiments (*p < 0.05, **p < 0.01, ***p < 0.001).