Figure 7.
Effect of OA and UA on UGT1A1 mRNA and protein in hPXR-silenced HepG2 cells. PXR mRNA and protein expression in HepG2 cells transfected with hPXR shRNA or hPXR shNC were determined by real-time Q-PCR analysis (A 1 ) and Western blotting analysis (B 1–C 1 ), respectively. The HepG2 cells transfected with hPXR shRNA were treated with OA (10, 20, and 40 μM) (A 2–C 2 ) and UA (10, 20, and 40 μM) (A 3–C 3 ) for 24 h. UGT1A1 mRNA and protein expression in hPXR-silenced HepG2 cells treated with OA and UA were determined by real-time Q-PCR analysis (A) and Western blotting analysis (B, C), respectively. The determination of mRNA and nuclear protein was normalized to GAPDH and β-actin, respectively. DMSO (0.1%) was used as the negative control. RIF (10 μM) was used as the positive control. The data are expressed as the mean ± SD of triplicate independent experiments (*p < 0.05, ***p < 0.001).