Figure 8.
Effect of OA and UA on UGT1A1 mRNA and protein in hCAR-silenced HepG2 cells. CAR mRNA and protein expression in HepG2 cells transfected with hCAR shRNA or hCAR shNC were determined by real-time Q-PCR analysis (A 1 ) and Western blotting analysis (B 1–C 1 ), respectively. Then the HepG2 cells transfected with hCAR shRNA were treated with OA (10, 20, and 40 μM) (A 2–C 2 ) and UA (10, 20, and 40 μM) (A 3–C 3 ) for 24 h. UGT1A1 mRNA and protein expression in hCAR-silenced HepG2 cells treated with OA and UA were determined by real-time Q-PCR analysis (A) and Western blotting analysis (B–C), respectively. The determination of mRNA and nuclear protein was normalized to GAPDH and β-actin, respectively. DMSO (0.1%) was used as the negative control. CITCO (10 μM) was used as the positive control. The data are expressed as the mean ± SD of triplicate independent experiments (**p < 0.01, ***p < 0.001).