Skip to main content
. 2019 Sep 25;25:7202–7208. doi: 10.12659/MSM.916886

Figure 3.

Figure 3

siRNA transfection and Transwell migration assay. (A, B) Western blot analysis was performed to test HIF-1α protein expression culturing in hypoxia after silencing HIF-1α. (C) RT-PCR was used to detect the effects of HIF-1α knockdown on the expression of HIF-1α mRNA levels in A2780 cells under hypoxic conditions. (F) After transfected with HIF-1α siRNA, MMP13 mRNA was downregulated. (D, E) MMP13 protein had the same change. The data are presented as the mean ± standard deviation. * p<0.05 versus normoxia group or the control siRNA group. (G–J) Suppressed effects of HIF-1α siRNA on the invasion potency of ovarian cancer A2780 cells. Invaded cells were counted in 5 microscopic fields per well. * p<0.05 versus the normoxia group. # p<0.05 versus the untreated group and the control siRNA group.