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. 2019 Sep 23;25:7149–7157. doi: 10.12659/MSM.917562

Figure 4.

Figure 4

MiR-34a over-expression inhibits biological activity of breast cancer cells by targeting CXCL10. (A) Putative binding sites of miR-34a within the CXCL10 predicted by bioinformatics tools. (B) Luciferase reporter assay was performed to validate relationship between CXCL10 and miR-34a. (C, D) CCK-8 assay and colony formation assay was conducted to evaluate the cell proliferation of breast cancer cells transfected with miR-34a mimics or mimics-NC. (E, F) Transwell assay assessed the migration and invasion abilities of breast cancer cells transfected with miR-34a mimics or mimics-NC. (G, H) The mRNA and protein expression of apoptotic and EMT related genes in breast cancer cells transfected with miR-34a mimics or mimics-NC. (I) The protein levels of CXCL10 in BT-474 and MDA-MB-231 cells transfected with miR-34a mimics or mimics-NC. Data are presented as the mean±standard deviation. ** P<0.01, * P<0.05 versus control group. CCK-8 – Cell Counting Kit-8; NC – negative control; EMT – epithelial-mesenchymal transition.