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(A–D) Establishment of the rat model of polycystic ovary syndrome (PCOS). The rats in the PCOS model group showed macroscopic appearances of PCOS in the ovaries (A), and histologically on hematoxylin and eosin (H&E) staining (B). Raised serum testosterone and glucose levels, which are also a feature of PCOS, were also detected. Data are presented as the mean ± standard deviation (SD). ** P<0.01, *** P<0.001 versus the control group.
