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. 2019 Sep 27;9:992. doi: 10.3389/fonc.2019.00992

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Copyright © 2019 Kalli, Voutouri, Minia, Pliaka, Fotis, Alexopoulos and Stylianopoulos.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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The growth of brain cancer MCS is hindered by a surrounding agarose matrix. (A,B) Multicellular spheroids (MCS) composed by H4 (A) or A172 (B) cells, were embedded in 1% agarose matrix or in free suspension and grew for 21 days. Images were taken every 2–3 days with an optical microscope and the area of each spheroid was quantified using ImageJ. The average % difference in each spheroid size was calculated and plotted for each cell line ±SE (≥6 spheroids/condition; 2 independent experiments; number of total replicates n = 12–18). Error bars indicate standard error (SEM). Asterisks (*) indicate a statistically significant difference between free spheroids and spheroids embedded in 1% agarose matrix. Scale bar: 0. 15 mm.