Figure 2.

Growth of Z. mobilis bearing pRLpfkA. (A) Cultures were inoculated from glycerol stocks of slow or fast growing isolants of ZM4 bearing pRLpfkA and were grown overnight, aerobically, in rich medium with spectinomycin and indicated concentrations of IPTG. Average OD₆₀₀ and standard deviations are from three biological replicates. (B) Whole cell lysates from overnight cultures in (A) were analyzed by Western blot. Equivalent of 1 ml at OD₆₀₀ = 0.006 per lane was analyzed. Proteins were detected with anti-FLAG antibody. A representative blot is shown.