Table 1.
Antibodies and Their Conditions Used for Each Assay
| Experiment | Antibody | Clone | Dilution | Company | Catalog No. |
|---|---|---|---|---|---|
| Adenovirus infectious assay | |||||
| Adenovirus hexon | 1:500 | Millipore | AB1056 | ||
| (Biotinylated anti-goat IgG (H+L) | 1:500 | Vector Lab | BA-5000 | ||
| Western blot | |||||
| Adenovirus fiber | 4D2 | 1:1000 | Thermo Fisher Scientific | MS-1027-P | |
| Anti-mouseGITRL/TNFSF18 | 721926 | 1:250 | R&D Systems | MAB21772 | |
| Anti-E1A (Ad2/Ad5) | M73 | 1:1000 | EMD Millipore | 05-599 | |
| α-Tubulin | B-5-1-2 | 1:1000 | Santa Cruz | sc-23948 | |
| GAPDH | 6C5 | 1:1000 | Santa Cruz | sc-32233 | |
| Goat anti-mouse IgG (H+L), HRP | 1:1500 | Thermo Fisher Scientific | 32430 | ||
| Goat anti-rat IgG (H+L), HRP | 1:10 000 | Thermo Fisher Scientific | 31470 | ||
| Flow cytometry | |||||
| Anti-mouse CD8a PE | 53–6.7 | 1:100 | eBiosciences | 12-0081-82 | |
| Anti-mouse CD4 eFluor® 450 | GK1.5 | 1:100 | eBiosciences | 48-0041-82 | |
| Anti-mouse CD3e FITC | 145-2C11 | 1:100 | eBiosciences | 11-0031-85 | |
| Anti-mouse CD45 APC efluor 780 | 30-F11 | 1:100 | eBiosciences | 47-0451-82 | |
| CD16/CD32, rat anti-mouse | 2.4G2 | 1:100 | BD Biosciences | BDB553141 | |
| Alexa Fluor® 647 rat anti-mouse GITRL | MIH44 | 1:100 | BD Biosciences | 563542 | |
| Alexa Fluor® 647 rat anti-mouse GITR | DTA-1 | 1:100 | BD Biosciences | 565151 | |
| Alexa Fluor® 647 rat IgG2b κ isotype | A95-1 | 1:100 | BD Biosciences | 557691 | |
| PE anti-mouse CD62L | MEL-14 | 1:100 | BioLegend | 104407 | |
| Brilliant Violet 605™ anti-mouse/human CD44 | IM7 | 1:100 | BioLegend | 103047 |