Figure 7.

Tau oligomers derived from brains exposed to single versus repetitive TBI show different seeding patterns and morphologies observed in Tau RD P301S FRET biosensor human cell line. Tau RD P301S FRET biosensor cells were exposed to a sub-lethal concentration (0.125 µM) of the oligomers alone or oligomers pre-incubated with T22 antibody (1:4 ratio oligomer to antibody) for 48 h in the presence of lipofectamine. All the oligomeric groups showed prominent seeding and unique aggregation patterns except the sham oligomers. Prominent seeding was not observed earlier than 48 h. (Merge scale bar: 10 µm, Zoomed Merge scale bar: 2 µm).