Table 2:
Reverse Transcription Mixture
| Component | Volume (μL) |
|---|---|
| Loading Reagent | 1.2 |
| 5X First-Strand Buffer | 11.2 |
| Dithiothreitol | 1.4 |
| SMARTer IIA Oligonucleotide | 5.6 |
| RNase Inhibitor | 1.4 |
| SMARTScribe Reverse Transcriptase | 5.6 |
| Total Volume | 32 |
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