Table 1.
Primers used for PCR amplification and sequencing.
| PDX CRC ID | Gene Symbol | Primer | Sequence 5’−−3’ |
|---|---|---|---|
| CRC269 and CRC269 R |
FAT4 | Forward | GTGTCTGTGGTTGAGAATGC |
| Reverse | TGGACCGGGTAGAAGACAGG | ||
| KMT2C | Forward | TGAAGAGTGGCTCCAGGAGA | |
| Reverse | CCTGTTCTAGCTGTTTCTGAACC | ||
| PIK3CA (for c.2176) | Forward | ACAGTTAGCCAGAGGTTTGG | |
| Reverse | AAACAACTCTGCCCCACTGC | ||
| PIK3CA (for c.3140) | Forward | AGCTATTCGACAGCATGCCA | |
| Reverse | GGTCTTTGCCTGCTGAGAGT | ||
| KMT2D (for c.2438) | Forward | CTATGCGCTGTGCCTGAGG | |
| Reverse | CTTCTCAAGCTCAGGGGAC | ||
| KMT2D (for c.7411) | Forward | GTTACCCCTCGCTTCCAGTC | |
| Reverse | CTTGGGACCTTGGCATGGAG | ||
| FOXA1 | Forward | GGCTTCTTCACTCGCTGTCT | |
| Reverse | CTACTGCGCCGGGACTCAG | ||
| SYNE1 | Forward | TGTTGTGGGGTTTCATTTCGT | |
| Reverse | TGCATTTTCCCTGGCTCACA | ||
| ZFR2 | Forward | GGGGCTTTGGTGTGTGTTTG | |
| Reverse | GTAGGTAGCCATGGTGGTGG | ||
| ADCY2 | Forward | GGAGGCTCTTAGAAACCAGAA | |
| Reverse | CCTGGTGGGATGTGGAAAGT | ||
| CRC293 | ARID1A | Forward | GCCCTGAACAATAACCTCACG |
| Reverse | GGTTGCCCGAAGCCGTAG | ||
| TP53 (for c.626) | Forward | GCTGGGGCTGGAGAGACG | |
| Reverse | GCACCACCACACTATGTCGA | ||
| TP53 (for c.743) | Forward | TGGCTCTGACTGTACCACCA | |
| Reverse | CTG GAG TCT TCC AGT GTG ATG | ||
| SYNE2 | Forward | TTGGCGTCTCTCAGAACAGC | |
| Reverse | CGATGTTTCACAGCTGGAACA | ||
| EP300 | Forward | TGGTGATTCCAGTCTGAATGAGT | |
| Reverse | ACAAATCCGGAGCTAGCCAC | ||
| CDKN2A | Forward | GCTTCCTTTCCGTCATGCC | |
| Reverse | TGGAAGCTCTCAGGGTACAA | ||
| MTOR | Forward | TGATGAACTTCGAAGCTGTGC | |
| Reverse | CTCAGTGACCTTCTTCTGCA | ||
| CRC382 | ATM | Forward | CTCAAACTATTGGGTGGATTTGT |
| Reverse | TCGTTTGCGAGAAGTGTCGA | ||
| TP53 (for c.610) | Forward | GCTGGGGCTGGAGAGACG | |
| Reverse | GCACCACCACACTATGTCGA | ||
| TP53 (for c.25) | Forward | TGCTGGATCCCCACTTTTCC | |
| Reverse | AGACAAGAGCAGAAAGTCAGTC | ||
| KLF5 | Forward | CGTTGTCACAGGTGAAAAGCC | |
| Reverse | GTGGTCAGAGCGCGAGAAG | ||
| MERTK | Forward | TGTGTGTGTATGTGTGTGTGT | |
| Reverse | ATGCTGCAATTCCTGAACGG | ||
| MYH10 | Forward | CTGTGTACGTATGTAATAGGGGCA | |
| Reverse | CCACTTGAAAATCCAAAATATGCTTCT | ||
| NUP98 | Forward | GCCTAGTCCCTCGTGAAAAGTC | |
| Reverse | GCATCAAGAAATGTGACTCACGG | ||
| SETD2 | Forward | GCCTATGTGGATCCCAGCAA | |
| Reverse | GCACTGGCAAGACAGCAAC |