Table 2.

Mutation statuses of 20 genes in the CRC lines in comparison with PDX tumors.

PDX CRC ID	Bladder PDX ID	PDX CRC ID	Gene Symbol	Transcript ID	CDS mutation (in PDX)	AA Mutation (in PDX)	CDS mutation (in PDX CRCs)
CRC958-1	BL0269F404P0	CRC269	FAT4	NM_001291303.1	c.1855C>T	p.R619C	c.1855C>T
			KMT2C	NM_170606.2	c.9694G>T	p.E3232★	c.9694G>T
			PIK3CA	NM_006218.2	c.2176G>A	p.E726K	c.2176G>A
					c.3140A>G	p.H1047R	c.3140A>G
			KMT2D	NM_003482.3	c.2438C>T	p.P813L	c.2438C>T
					c.7411C>T	p.R2471★	c.7411C>T
			FOXA1	NM_004496.3	c.150G>A	p.M50I	c.150G>A
			SYNE1	NM_182961.3	c.2653T>G	p.L885V	c.2653T>G
			ZFR2	NM_015174.1	c.172G>A	p.G58S	c.172G>A
			ADCY2	NM_020546.2	c.439G>T	p.V147L	c.439G>T
CRC958-2	BL0269F404P0_GDC-R	CRC269-R	FAT4	NM_001291303.1	c.1855C>T	p.R619C	c.1855C>T
			KMT2C	NM_170606.2	c.9694G>T	p.E3232★	c.9694G>T
			PIK3CA	NM_006218.2	c.2176G>A	p.E726K	c.2176G>A
					c.3140A>G	p.H1047R	c.3140A>G
			KMT2D	NM_003482.3	c.2438C>T	p.P813L	c.2438C>T
					c.7411C>T	p.R2471 ★	c.7411C>T
			FOXA1	NM_004496.3	c.150G>A	p.M50I	c.150G>A
			SYNE1	NM_182961.3	c.2653T>G	p.L885V	c.2653T>G
			ZFR2	NM_015174.1	c.172G>A	p.G58S	c.172G>A
			ADCY2	NM_020546.2	c.439G>T	p.V147L	c.439G>T
CRC958-3	BL0293F563P0	CRC293	ARID1A	NM_006015.4	c.413C>G	p.S138★	c.413C>G
			TP53	NM_001126112.2	c.626G>A	p.R209Q	c.626G #
					c.743G>A	p.R248Q	c.743G>A
			SYNE2	NM_015180.4	c.4177C>T	p.R1393W	c.4177C>T
			EP300	NM_001429.3	c.4040G>T	p.G1347V	c.4040G>T
			CDKN2A	NM_000077.4	c.442G>A	p.A148T	c.442G>A
			MTOR	NM_004958.3	c.5533G>A	p.E1845K	c.5533G>A
CRC958-4	BL0382F1232P0	CRC382	ATM	NM 000051.3	c.5557G>A	p.D1853N	c.5557G>A
			TP53	NM_001126112.2	c.610G>T	p.E204★	c.610G>T
					c.25G>T	Sense mutation	c.25G>T
			KLF5	NM_001730.4	c.1255G>C	p.E419Q	c.1255G>C
			MERTK	NM_006343.2	c.878G>A	p.R293H	c.878G>A
			MYH10	NM_001256012.1	c.2879C>G	p.S960C	c.2879C>G
			NUP98	NM_016320.4	c.3424C>G	p.Q1142E	c.3424C>G
			SETD2	NM_014159.6	c.6694C>T	p.P2232S	c.6694C>T

^★STOP codon

^#no mutataion observed