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. Author manuscript; available in PMC: 2019 Dec 1.
Published in final edited form as: AIChE J. 2018 Jun 11;64(12):4182–4186. doi: 10.1002/aic.16324

Figure 2.

Figure 2.

The pyr cluster is responsible for the biosynthesis of pyranonigrin A. (A) The pyr cluster identified from P. thymicola IBT 5891. orf1 encodes IMPDH, pyrA encodes PKS-NRPS, pyrB encodes P450, pyrC encodes flavin-dependent monooxygenase, pyrD encodes hydrolase, orf2 encodes flavin binding oxidoreductase, orf3 encodes GMC oxidoreductase. (B) Characterization of pyrA in A. nidulans A1145. Shown are chromatograms of extracts from: i) A. nidulans expressing pyrA; ii) A. nidulans containing the control vector. (C) Reconstitution of pyr cluster in A. nidulans A1145. Shown are chromatograms of extracts from: i) A. nidulans expressing the putative pyr cluster; ii) A. nidulans containing control vectors.