Table 3.
Mediation analysis of CML and CEL with cardiovascular mortality through hs-CRP, thiols, and sVCAM-1
| Predictor | Potential Mediator | Effect (Path)a | Coefficient (95% CI, bc)b | Proportion Mediated (95% CI, bc)bc |
|---|---|---|---|---|
| CML | hs-CRP | Indirect effect (ab path) | −0.01 (–0.04 to <0.001) | Not mediated |
| Total effect (ab+c’ path) | 0.15 (0.04 to 0.26) | |||
| Thiol | Indirect effect (ab path) | 0.04 (0.02 to 0.06) | 20% (9% to 61%) | |
| Total effect (ab+c’ path) | 0.17 (0.06 to 0.29) | |||
| sVCAM-1 | Indirect effect (ab path) | 0.02 (0.003 to 0.08) | 17% (1% to 39%) | |
| Total effect (ab+c’ path) | 0.15 (0.04 to 0.25) | |||
| CEL | hs-CRP | Indirect effect (ab path) | −0.007 (–0.03 to 0.005) | Not mediated |
| Total effect (ab+c’ path) | 0.13 (0.05 to 0.23) | |||
| Thiol | Indirect effect (ab path) | 0.02 (0.003 to 0.04) | 12% (6% to 36%) | |
| Total effect (ab+c’ path) | 0.16 (0.08 to 0.26) | |||
| sVCAM-1 | Indirect effect (ab path) | 0.03 (0.006 to 0.07) | 21% (2% to 40%) | |
| Total effect (ab+c’ path) | 0.13 (0.05 to 0.22) |
CML, Nε-(Carboxymethyl)lysine; CEL, Nε-(Carboxyethyl)lysine; hs-CRP, high sensitivity C-reactive protein; sVCAM-1, soluble vascular cell adhesion molecule-1; 95% CI, 95% confidence interval; Bc, bias corrected.
The coefficients of the indirect ab path and the total ab+c’ path are standardized for the SD of the potential mediators, circulating CML and CEL concentrations and cardiovascular mortality.
95% CIs for the indirect and total effects, and proportion mediated were bias-corrected after running 2000 bootstrap samples.
The size of the significant mediated effect is calculated as the standardized indirect effect divided by the standardized total effect multiplied by 100.