Table I.
Gene | Sequences |
---|---|
Type 1 collagen | |
Forward | 5′-AGACTGGCAACCTCAAGAAG-3′ |
Reverse | 5′-TTCGGTTGGTCAAAGATAAA-3′ |
Type 3 collagen | |
Forward | 5′-ATGGTTGCACGAAACACACT-3′ |
Reverse | 5′-CTTGATCAGGACCACCAATG-3′ |
MMP-1 | |
Forward | 5′-CTGGCCACAACTGCCAAAT-3′ |
Reverse | 5′-CTGTCCCTGAACAGCCCAGTACTTA-3′ |
MMP-2 | |
Forward | 5′-TCTCCTGACATTGACCTTGGC-3′ |
Reverse | 5′-CAAGGTGCTGGCTGAGTAGATC-3′ |
MMP-3 | |
Forward | 5′-ACACCGGATCTGCCAAGAGA-3′ |
Reverse | 5′-CTGGAGAACGTGAGTGGAGTCA-3′ |
MMP-9 | |
Forward | 5′-TTGACAGCGACAAGAAGTGG-3′ |
Reverse | 5′-GCCATTCACGTCGTCCTTAT-3′ |
TIMP-1 | |
Forward | 5′-TCTGCAATTCCGACCTCGTCATCA-3′ |
Reverse | 5′-AAGGTGGTCTGGTTGACTTCTGGT-3′ |
Tropoelastin | |
Forward | 5′-CGGAATTCACCTCTTAAGCCAGTTCCCG-3′ |
Reverse | 5′-CCCAAGCTTCGGGAACACCTCCGACACTA-3′ |
GAPDH | |
Forward | 5′-AGGGCTGCTTTTAACTCTGGT-3′ |
Reverse | 5′-CCCCACTTGATTTTGGAGGGA-3′ |
MMP, matrix metalloproteinase; TIMP, tissue inhibitor of metalloproteinase.