Figure 4.

IL-21 treatment inhibits the proliferation, invasion and migration of A549 cells. (A) Cell proliferation was measured using a colony formation assay. (B) Cell proliferation was also assessed by Cell Counting Kit-8 assay. (C) Representative images and (D) quantification of cell invasion, assessed using Transwell assays (magnification, ×200). (E) Representative images and (F) quantification of cell migration, assessed using a scratch assay (magnification, ×4). *P<0.05, ^**P<0.01 and ^***P<0.001 vs. control (vehicle-treated). IL, interleukin.