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. 2019 Sep 17;44(5):1677–1686. doi: 10.3892/ijmm.2019.4344

Figure 4.

Figure 4

Effects of incremental load training on the expression of TGF-β1 and TAK1 in the kidney tissues of aged mice. (A) Western blot analysis was used to determine the protein expression of TGF-β1 and TAK1 in the three groups. Bar graphs show the relative quantification of (B) TGF-β1 and (C) TAK1. (D) Expression of TAK1 was assessed by immunohistochemical staining (magnification, ×200; scale bar=20 μm). The brown color indicates a positive signal. (E) Relative OD values of TAK1 staining are shown in a bar graph. Values are expressed as the mean ± standard deviation (n=3). *P<0.05, **P<0.01. YC, young control group; OC, elderly control group; OY, elderly exercise group; TGF, transforming growth factor; OD, optical density; TAK1, TGF-β-activated kinase 1.