Figure 2.

Proliferation and apoptosis features of the 3-dimensional liver microsphere tissue culture model. (A-a) The freshly recovered mouse liver sift80 microsphere tissue was cultured in 2% BCS/DMEM for 3, 7 and 14 days, and were then subjected to (a) H&E staining, (b) Hoechst 33258 staining and (c) immunohistochemical staining for PCNA or c-Myc. (B) IF staining for PCNA or c-Myc. (a) Sudan Black B (5%) treatment was used to significantly decrease auto-fluorescence. (b) The cultured sift80 microsphere tissue were subjected to IF staining for either PCNA or c-Myc at the indicated time points, followed by blocking with 5% Sudan Black B to eliminate background fluorescence. Control IgG was used as a negative control. Each assay condition was performed in triplicate. Representative results are presented. H&E, hematoxylin and eosin; DMEM, Dulbecco's modified Eagle's medium; PCNA, proliferating cell nuclear antigen; c-Myc, anti-Myc proto-oncogene protein; IF, immunofluorescence.