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. 2019 Aug 21;44(5):1653–1666. doi: 10.3892/ijmm.2019.4321

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Copyright: © Zhu et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Quantitative analysis of the effects of liver injury-inducing drugs on hepatotoxicity-associated genes. The 4 week-old C57BL/6 male mice (n=5/group) were injected intraperitoneally daily with azithromycin (12 mg/kg), paracetamol (10 mg/kg), levofloxacin (10 mg/kg) or DMSO for 24 or 72 h. Concomitantly, subconfluent MPH and freshly isolated sift80 microsphere tissues were treated with azithromycin (125 nM), paracetamol (100 μM), levofloxacin (5 μM) or DMSO for 24 or 72 h. Total RNA was isolated from (a) the microsphere tissue, (b) mouse liver tissues and (c) the MPH treated with (A) azithromycin, (B) paracetamol and (C) levofloxacin, and subjected to touchdown quantitative polymerase chain reaction analysis of 5 hepatotoxicity-associated genes. All samples were normalized to respective GAPDH expression levels. Each assay condition was performed in triplicate. ^**P<0.01 vs. DMSO group. MPH, mouse primary hepatocytes.