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. 2019 Oct 4;14(10):e0223420. doi: 10.1371/journal.pone.0223420

Fig 1. The effects of the AhR-targeted siRNA transfection on AhR mRNA expression (mean ± SEM) in porcine granulosa cells.

Fig 1

To confirm the silencing of AhR gene in porcine granulosa cells, the expression of AhR was determined in: 1/ untransfected (UTR) cells, 2/ cells transfected with irrelevant siRNA sequence (TRNEG) and 3/ cells transfected with the three relevant siRNAs (TR). After 24 hours of incubation (with pure medium, medium with irrelevant siRNAs or medium with relevant siRNAs), cells were cultured for 3, 12 or 24 h (four biological replicates/time point). AhR mRNA expression level was examined by qRT- PCR and expressed as arbitrary units (AU). mRNA expression level in UTR group was considered as “1”, and the mRNA expression level in TRNEG and TR groups was expressed as a fraction of the UTR value. Stasistical analysis (one-way ANOVA followed by Tukey test) was performed on raw data. Different superscripts designate statistical significant differences (p<0.05) between untransfected UTR, TRNEG and TR groups.