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. 2019 Aug 22;4(16):e129299. doi: 10.1172/jci.insight.129299

Figure 3. Assessing MALAT1 lncRNA expression in the validation (islet) and prediction (pancreas) sample sets.

Figure 3

The 3 categorized human islet sample groups in the validation set (n = 75) were assessed for (A) insulin content levels, (B) (pro-) insulin transcript levels, and (C and D) MALAT1 lncRNA expression (using qPCR primer/probe assay Hs_00273907 and Hs_01910177, respectively) targeting 2 variants of MALAT1 lncRNA. To test the difference between the 3 categorized groups, nonparametric, 1-way ANOVA was used, and significant P values, adjusted for multiple comparisons, are reported. Each dot in the violin plot represents a different islet sample preparation. Horizontal solid blue line represents the median for each group, the horizontal dotted line represents quartiles, and the polygons represent the density of individual data points and extend to min/max values. FoD, fold over the detectable limit (55). (E) MALAT1 lncRNA qPCR was carried out before islet isolation. (F) ROC curve for MALAT1 lncRNA of pancreas tissue samples (n = 19) to stratify postisolation islet quality (group 1 vs. group 3).