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. 2019 Aug 22;4(16):e126982. doi: 10.1172/jci.insight.126982

Figure 2. UC tissue stromal cell characterization.

Figure 2

(A) Using flow cytometry, we identified a population of stromal cells from the umbilical cord arteries that were CD45–, Ter119–, and CD31–. Of this population, approximately 57.8% were CD51+ and PDGFRα–, 8.57% were CD51+ and PDGFRα+, and 32.7% were CD51– and PDGFRα –. (B) Immunofluorescent-stained umbilical cord sections for CD31, CD51, and PDGFRα revealed a perivascular location around the umbilical artery for CD51+ and PDGFRα+ cells (marked by yellow arrowhead). Scale bar: 50 μm. (C) Using flow cytometry, CD51+PDGFRα+ cells were positive for various cell surface markers associated with MSCs (CD105, CD73, CD90, Stro-1, CD44, CD271, CD146, and PDGFRβ) and early embryonic cells (SSEA4 and GD2). (n = 3–4). (D) cMSCs expressed Nestin as shown by real-time PCR (n = 3–4). (E) cMSCs were enriched for a number of HSC maintenance genes, similar to BM MSCs (n = 4). *P ≤ 0.05; **P ≤ 0.01; ***P ≤ 0.001. Two-tailed t tests were performed.