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. 2019 Aug 22;4(16):e126982. doi: 10.1172/jci.insight.126982

Figure 5. Functional platelet characterization, showing activation in response to ADP stimulation.

Figure 5

(A–D) Flow cytometry plots of PAC-1 expression in (A) unstimulated platelets from the control group and (B) unstimulated platelets from the cMSC group compared with (C) stimulated platelets from the control group and (D) stimulated platelets from the cMSC coculture group. (E) Quantification of the fold change in PAC-1 expression among the various groups (n = 3). A quantitative flow cytometry assay was performed, measuring the ability of platelets from the cMSC coculture group to adhere to matrix proteins bound to beads. (F) Platelets stimulated with ADP were able to bind efficiently to matrix-coated beads (n = 3–4). (G) Bound stimulated platelets were activated and expressed CD62P to a greater extent than unstimulated bound platelets (n = 3–4). (H) Fold change of stimulated CD62P+ platelets bound to matrix-coated beads relative to unstimulated platelets (n = 3–4). *P ≤ 0.05; **P ≤ 0.01; ****P ≤ 0.0001. E was analyzed by 1-way ANOVA with Tukey post hoc test. F–H was analyzed by 2-tailed t tests.